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Macranthoidin B downregulated epithelial-mesenchymal transition and COX-2/PGE 2 pathway in endometriotic tissues. (A–D) The mRNA levels of Cdh1 , Cdh2 , Vim , Twist , Slug , Snail , and Zeb1/2 were measured by RT-qPCR. (E–G) The protein levels of E-cadherin, N-cadherin, and Vimentin were detected by Western blotting assay. (H) Detection of E-caderin and Vimentin in ectopic tissue by <t>immunofluorescence</t> assay. (I) The gene expression of Ptgs2 and Ptges were tested by RT-qPCR. (J–L) The contents of COX-2 and PGE 2 were analyzed by Western blotting and ELISA assays. # P < 0.05 to control, ## P < 0.01 to control, * P < 0.05 to EMS ** P < 0.01 to EMS. Columns, mean (n = 3). Bars, SD. Scale bar = 100 μm. EMS, endometriosis.
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Macranthoidin B downregulated epithelial-mesenchymal transition and COX-2/PGE 2 pathway in endometriotic tissues. (A–D) The mRNA levels of Cdh1 , Cdh2 , Vim , Twist , Slug , Snail , and Zeb1/2 were measured by RT-qPCR. (E–G) The protein levels of E-cadherin, N-cadherin, and Vimentin were detected by Western blotting assay. (H) Detection of E-caderin and Vimentin in ectopic tissue by immunofluorescence assay. (I) The gene expression of Ptgs2 and Ptges were tested by RT-qPCR. (J–L) The contents of COX-2 and PGE 2 were analyzed by Western blotting and ELISA assays. # P < 0.05 to control, ## P < 0.01 to control, * P < 0.05 to EMS ** P < 0.01 to EMS. Columns, mean (n = 3). Bars, SD. Scale bar = 100 μm. EMS, endometriosis.

Journal: Frontiers in Pharmacology

Article Title: Macranthoidin B restrains the epithelial-mesenchymal transition through COX-2/PGE 2 pathway in endometriosis

doi: 10.3389/fphar.2024.1492098

Figure Lengend Snippet: Macranthoidin B downregulated epithelial-mesenchymal transition and COX-2/PGE 2 pathway in endometriotic tissues. (A–D) The mRNA levels of Cdh1 , Cdh2 , Vim , Twist , Slug , Snail , and Zeb1/2 were measured by RT-qPCR. (E–G) The protein levels of E-cadherin, N-cadherin, and Vimentin were detected by Western blotting assay. (H) Detection of E-caderin and Vimentin in ectopic tissue by immunofluorescence assay. (I) The gene expression of Ptgs2 and Ptges were tested by RT-qPCR. (J–L) The contents of COX-2 and PGE 2 were analyzed by Western blotting and ELISA assays. # P < 0.05 to control, ## P < 0.01 to control, * P < 0.05 to EMS ** P < 0.01 to EMS. Columns, mean (n = 3). Bars, SD. Scale bar = 100 μm. EMS, endometriosis.

Article Snippet: For immunofluorescence staining, rabbit anti-E-cadherin or anti-Vimentin antibodies (1:200 dilution; Proteintech, China) were applied to the endometrial tissue.

Techniques: Quantitative RT-PCR, Western Blot, Immunofluorescence, Gene Expression, Enzyme-linked Immunosorbent Assay, Control